Alejandro Bertolo studied "suiboku" and "nihonga" painting (two forms of Japanese classical painting) with the Japanese artists Tazuko NIIMURA, Sho FUNAKI and Hiroshi YAMAMOTO, and Japanese calligraphy with Master Calligrapher Masako Inkyo. He learned the Japanese techniques of mounting paintings as well as folding screen-making, with Akihiro KATO, Conservator of the Japanese Art Collection of the Freer and Sackler Galleries in Washington D.C.

Having developed a personal style, he paints wildlife, particularly those species threatened with extinction in their natural habitats.